Thy1-yfp mouse
WebbMammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown Gene/Insert Gene/Insert name Thy1 promoter Alt name Thy-1.2 Species M. musculus (mouse) Insert Size (bp) 6500 GenBank ID Entrez Gene Thy1 ( a.k.a. CD90, T25, Thy-1, Thy-1.2, Thy1.1, … WebbThy1-YFP mice were utilized to observe the morphology of the dendritic spines in the penumbra area. MEC17 is the specific acetyltransferase of α-tubulin. Thy1 Cre ERT2-eYFP and MEC17 fl/fl mice were mated to produce mice with decreased expression of α-Ac-Tub in dendritic spines of pyramidal neurons in the cerebral cortex.
Thy1-yfp mouse
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Webb20 juli 2016 · Thresholds in male mice were comparable with those in females (method A 2: 3 to 5 seconds; n = 6; P = 0.19, Mann–Whitney U test), so for subsequent experiments data from male and female mice were pooled. Control experiments in Thy1/ChR2-YFP mice showed that CSD was not induced by pulses of red (630 nm) light that do not activate … Webb13 okt. 2024 · Given this versatility, it your inexpensive to believe is the methods featured here could work on any strain of phosphorus protein-expressing mouse or rat, as well as other indicators (e.g., iGluSnFr in Ai85 mice) or optogenetic fusion proteins (e.g., Thy1-ChR2-YFP) or in non-transgenic animals with brain-wide infection by viruses that like …
4T1 cells were injected subcutaneously (s.c.) either in the mammary fat pad of the flank, or s.c. into the ear pinna of the BALB/c(Thy1-YFP) or BALB/c mice. The orthotopically growing 4T1 tumour line forms metastases in the lung, while other organs are mostly metastasis-free. To obtain mice with … Visa mer The JAX mouse strain B6.Cg-Tg(Thy1-YFP)16Jrs/J was obtained from The Jackson Laboratory. The mice were kept in a conventional animal house. … Visa mer All experimental protocol were performed in accordance with national (1998. XXVIII; 40/2013) and European (2010/63/EU) animal ethics guidelines. The were … Visa mer The 4T1 mouse mammary carcinoma cell line was obtained from ATCC and kept in RPMI 1640 tissue culture medium supplemented with 10% FCS (Lonza, Basel, … Visa mer The photographs were made with a Nikon Eclipse E600 fluorescent microscope, using the B-2A filter block (EX:450; DM > 505; BA:520). The micrographs were made … Visa mer WebbRecently, a ratiometric CFP-YFP based construct, termed “Cl-Sensor”, with a relatively high sensitivity to Cl has been proposed (Markova et al., 2008). In the present study, we have developed conditions for the efficient expression of Cl-Sensor in tiny neuronal compartments including distal dendrites and spines.
Webb3 apr. 2024 · 未经历重组的细胞可通过截短YFP的免疫染色检测到。 对于Thy1-Brainbow小鼠，一些神经元可表达多达16个拷贝的转基因，而每个拷贝在重组后激活不同荧光蛋白 … WebbIn vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice. Exp Eye Res. 2016;146:137–144. 42. Li C, Yang M, Wang X, et al. Glutazumab, a novel long-lasting GLP-1/anti-GLP-1R antibody fusion protein, exerts anti-diabetic effects through targeting dual receptor binding sites.
Webb12 aug. 2024 · A transgenic construct containing a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized …
WebbSpinal cord injury (SCI) causes long-term disability and has no effective clinical treatment. After SCI, extracellular adenosine triphosphate (ATP) leads to an influx of extracellular Ca 2+, and this Ca 2+ overload causes neuronal toxicosis and apoptosis. The biological functions of leptin have been widely investigated in the central nervous system. shortcut key for deleted tabWebb27 okt. 2014 · The B6.Cg-Tg (Thy1-YFP)16Jrs/J transgenic mouse strain, widely used to study neuronal development and regeneration, expresses the yellow fluorescent protein … sandy\u0027s pet food center concord nhWebb6 mars 2015 · The Thy1.2 YFP-16 mouse expresses yellow fluorescent protein (YFP) in specific subsets of peripheral and central neurons. The original characterization of this … sandy\u0027s pet center concord nhWebb11 apr. 2024 · ( A,B) MIPs of image stacks (580×580×80 µm 3) of a Thy1-YFP-16 retina, measured ( A) without and ( B) with AO, respectively, normalized to AO images. Red asterisk: center of a 19×19 µm 2 WS area. Top: … see more Figure 3—source data 1 Source image stacks of retinal neurons ( Figure 3A and B ). shortcut key for delete in laptopWebbFigure 1.11: Non-invasive imaging of sciatic nerve regeneration in thy-1-YFP-16 mice. D: regeneration through a nerve guide at 2-weeks postinjury; E: at 4-weeks post-injury; H: … shortcut key for delete sheet in excelWebbthat no YFP fluorescent signal was detected outside of the nervous system in these transgenic mice, but Thy1- activating conditions have not been systematically studied in … shortcut key for delete row in excelWebb1 juli 2024 · Thy1-YFP Neurofluorescent transgenic mice Nestin Nerve Abbreviations 5-HT 5-Hydroxytryptamine (serotonin) ACh Acetylcholine Akt Protein kinase B (PKB) Mapttm1 … sandy\\u0027s paradise infinity resort