T4 dna ligase neb
WebE-mailadres [email protected] 1.4. Telefoonnummer voor noodgevallen Europa +1 978-380-2125 _____ Pagina 1 / 10 Adres leverancier New England BioLabs 240 County Road Ipswich, MA 01938 USA ... E6288 - T4 DNA Ligase Datum van herziening 25-mrt-2024 RUBRIEK 5: Brandbestrijdingsmaatregelen 5.1. Blusmiddelen WebConvenient master mix formats Extreme purity with NEB’s T4 DNA Ligase Equivalent amounts of protein were loaded and silver stained using SilverXpress™. Marker M is NEB’s Broad Range Protein Marker (NEB #P7702). Available Products Further information can be found in our Technical Resources section or at neb.com Cloning / Synthetic Biology
T4 dna ligase neb
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WebConvenient master mix formats Extreme purity with NEB’s T4 DNA Ligase Equivalent amounts of protein were loaded and silver stained using SilverXpress™. Marker M is … WebNEBioCalculator Ligation Calculator This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. …
WebApr 6, 2024 · NEB and Promega T4 ligase buffers are 10×, allowing more DNA volume in a small reaction. NEB T7 ligase buffer is 2×, allowing 4 µL DNA volume in a typical 10 µL rxn with 1 µL total enzyme. T3 ligase has a much higher salt tolerance, better resisting salt contamination in DNA samples (max 250 mM). Golden Gate Enhancer WebNEB # PRODUCT VOLUME E6041A NEBNext End Repair Enzyme Mix 0.06 ml E6042A NEBNext End Repair Reaction Buffer 0.12 ml E6047A Quick T4 DNA Ligase 0.06 ml E6048A NEBNext Quick Ligation Reaction Buffer 0.12 ml E6030A Bst DNA Polymerase, Large Fragment 0.03 ml E6035A NEBNext Adaptor Fill-in Reaction Buffer 0.05 ml …
WebMultiplex (NEB #E7335, #E7500, #E7710, #E7730, #E6609 or #E7600) Oligos for Illumina. ... Ligase, 8 nmol 12 bp adapter, and 1X T4 DNA Ligase Buffer incubated at 16°C overnight results in no detectable unligated adapter as determined by agarose gel electrophoresis. Functional Activity ... WebT4 DNA Ligase Buffer (10X) 2 µl NEB Golden Gate Enzyme Mix (BsaI-HFv2) 1–2 µl (5) Nuclease-free H 2 O to 20 µl (6) (1) Or user provided. (2) Precloned inserts must possess BsaI restriction sites at both ends of the insert sequence and in the proper orientation.
Webwww.neb.com [email protected] New England Biolabs Certificate of Analysis M0202L / Lot: 10183117 Page 1 of 3 Product Name: T4 DNA Ligase Catalog Number: M0202L Concentration: 400,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of 6 µg of Lambda-HindIII DNA in 30 minutes at 16°C in a
WebThe volumes provided are sufficient for preparation of up to 50 reactions (NEB #E6270L). All reagents should be stored at –20°C. (green) NEBNext End Repair Enzyme Mix (green) NEBNext End Repair Reaction Buffer (red) T4 DNA Ligase (red) Bst 2.0 WarmStart DNA Polymerase (red) T4 DNA Ligase Buffer for Ion Torrent refrigiwear iron tuffWebE-mailadres [email protected] 1.4. Telefoonnummer voor noodgevallen Europa +1 978-380-2125 _____ Pagina 1 / 10 Adres leverancier New England BioLabs 240 County Road … refrigiwear recensioniWebT4 DNA Ligase Buffer (10X) 2 µl NEB Golden Gate Enzyme Mix (BsaI-HFv2) 1–2 µl (5) Nuclease-free H 2 O to 20 µl (6) (1) Or user provided. (2) Precloned inserts must … refrigiwear romaWebT4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky … refrigiwear salaryWebDec 28, 2024 · Reactions included 1 uM of the ligase and 100 nM of the substrate and T4 DNA ligase reaction buffer (50 mM Tris-HCl pH 7.5 @ 25°C, 1 mM ATP and 10 mM MgCl2) or NEBuffer 2 (10 mM Tris pH 7.9 @ 25°C, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT). refrigiwear resoWebNEB # PRODUCT VOLUME E6041A NEBNext End Repair Enzyme Mix 0.06 ml E6042A NEBNext End Repair Reaction Buffer 0.12 ml E6047A Quick T4 DNA Ligase 0.06 ml … refrigiwear retailersWebFor blunt ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 2 hours or 1 µl high concentration T4 DNA Ligase for 10 minutes. Alternatively, NEB's Quick Ligation Kit (#M2200S) [30 reactions] or (#M2200L) [150 … refrigiwear scalo milano