T2a-h2b-mcherry
WebJun 11, 2024 · We primarily constructed C- or N-terminal fusions or in some cases used the self-cleaving T2A peptide 22 (T2A-H2B-reporter) with different reporter tags such as GFP, … Web中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒,细菌,真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803
T2a-h2b-mcherry
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WebNov 29, 2024 · a Visualization of H2B locus using CRISPR-Tag_v2 (containing four or six repeat units), which was inserted into the intron of mCherry. H2B-mCherry was imaged and H2B loci were labeled by dCas9-GFP ... WebHuman (Homo sapiens) のCCNG1 (cyclin G1)遺伝子を含むベクター、レンチウイルス、アデノウイルス、 (AAV) アデノ随伴、アデノ随伴ウイルス、MMLV レトロウイルス,、piggyBac, shRNA、gRNA、 ガイドRNA、 CRISPR-Cas9 、クリスパー、プラスミド
WebThe H1-EFNB2-2A-mCherry reporter cells were pluripotent and could differentiate into all three germ layer lineages. Simultaneous expression of mCherry was observed when expression of EFNB2 was increased during endothelial cell differentiation. http://www.app17.com/c145959/products/d11302691.html
Web上游Cas9蛋白的C端将会添加一些额外的T2A残基(GSGEGRGSLLTCGDVEENPG),而下游Puro蛋白的N端将会有额外的脯氨酸。在T2A肽的N端加入GSG序列,可提高剪切效率。 pU6-gRNA-Cas9-T2A-Puro质粒为氨苄青霉素抗性。 pU6-gRNA-Cas9-T2A-Puro质粒图谱如下。 图2. pU6-gRNA-Cas9-T2A-Puro质粒图谱。 Web023139 B6;129S- Gt(ROSA)26Sor tm1.1Ksvo /J R26 LSL H2B mCherry (Rosa26-CAG-LSL-H2B-mCherry) mutant mice have a loxP -flanked STOP sequence followed by an H2B-mCherry fusion protein inserted into the Gt(ROSA)26Sor locus. Cre inducible, nuclear expression of the mCherry protein allows the monitoring of Cre activity in living tissues …
WebJan 7, 2024 · General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor. origin : 37. pi : alt_names: ... EasyFusion T2A-H2B-GFP 112851; EasyFusion Halo-mAID 112852; pR26-CAG-H2B-miRFP703 112853; pcDNA5-T/O-Ds1-mCherry 112855; questions and comments .
WebFruit fly Taf6 vectors (from $99), available in lentivirus, AAV, adenovirus, shRNA, CRISPR & many other formats from VectorBuilder. supermarket non woven bagWebDetailed high-resolution three-dimensional imaging of cells and nuclei from various somatic and meiotic cell types showed that H2B-mCherry produced remarkably clear images of … supermarket north andover maWebSender cells expressed either Dll1 or Dll4 along with a co-translational H2B-mCherry readout, under control of a 4epi-tetracycline (4epi-Tc)-induced promoter ( LeBon et al., 2014 , Sprinzak et al., 2010 ). We engineered receiver cells to express chimeric Notch1 receptors whose intracellular domain is replaced by the transcription factor Gal4 ( supermarket non woven bag factoriesWebKR20240031832A KR1020247044336A KR20247044336A KR20240031832A KR 20240031832 A KR20240031832 A KR 20240031832A KR 1020247044336 A KR1020247044336 A KR 1020247044336A KR 20247044336 A KR20247044336 A KR 20247044336A KR 20240031832 A KR20240031832 A KR 20240031832A Authority KR … supermarket no more weekly adWebFgfr1T2A-H2B-GFP; Fgfr2T2A-H2B-mCherry mice (Molotkov et al., 2024)were used to assess FGFR1 and FGR2 expression in the DG. Both males and females were used and showed no obvious differences; therefore, data for both were pooled. Two-month-old mutants and Cre– control litter-mates were administered an intraperitoneal injection of … supermarket night club zurich pic 2003WebMar 15, 2024 · HH-CAS-098lentiGuide-mCherry-T2A-Puro慢病毒sgRNA表达载体红色荧光嘌呤霉素. HH-CAS-097lentiGuide-copGFP-T2A-Puro慢病毒sgRNA表达载体绿色荧光嘌呤霉素. HH-CAS-096lentiGuide-Zeo慢病毒sgRNA表达载体博来霉素. HH-CAS-095lentiGuide-NEO慢病毒sgRNA表达载体新霉素 supermarket north andoverWebPlasmid PhyB-mCherry-Htb2 from Dr. Chao Tang's lab contains the insert PhyB and is published in Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub … supermarket o city of industry khai truong