WebpSB1C3 (Search Vector Database) Vector type Mammalian Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Chloramphenicol, 25 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name eGFP Species Synthetic Promoter None Cloning Information Cloning method Restriction Enzyme WebFor E. coli expression, we cloned the TMM construct into the standard high-copy BioBrick vector pSB1C3. For expression in C. striatum we used the vector pSEVA351, a universal vector with a high-copy replication origin ( Standard European Vector …
Team:Glasgow/Project/Mobility Proteins - 2014.igem.org
WebEngineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2024 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2024. WebBlue Cross Blue Shield of Massachusetts provides a Summary of Benefits and Coverage (SBC) with online access to the corresponding coverage policy to all of our fully insured members and accounts. To view your coverage policy document, select the size of your … es-wjx16-gy
A versatile bacterial expression vector based on the synthetic
WebJan 1, 2024 · We added attB and attP sites to the plasmids pSB1A2-BBa_K118014, pSB1C3-BBa_K118006, and pSB1C3-BBa_K118005 carrying crtE, crtB, and crtI, respectively, as shown in Supplementary Fig. S3. Then, five linearized plasmid segments ( Supplementary Fig. S3 ) containing four pairs of attB and attP DNA parts in direct orientation were prepared as ... WebContact Address:No. 2699, Qianjin Street, Changchun City, Jilin Province Mail:[email protected] Phone:0431-85168420 WebpSB1C3 (Search Vector Database) Backbone size w/o insert (bp) 2000 Total vector size (bp) 2700 Modifications to backbone Added T7 promoter upstream of GOI Vector type Synthetic Biology Growth in Bacteria Bacterial Resistance (s) Chloramphenicol, 25 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert eswl urology nhs