2024 Phospho-akt1/2/3 ser473 cellular kit

Phospho-akt1/2/3 ser473 cellular kit

Author: hlrc

August undefined, 2024

WebAKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. WebUp-regulation of O-GlcNAcylation increases Akt1 phosphorylation at Ser473. The 8305C cells were treated with 5 μM Thia-G or were transfected with 500 ng OGT plasmid, and then cell lysates were resolved by SDS-PAGE, and phospho-Akt1 (Ser473) or Akt1 were determined with specific antibodies (Figure 4A and B). An increase in Ser473 ...

O -GlcNAcylation enhances the invasion of thyroid anaplastic …

WebThe Total SHP1 cellular assay monitors the expression level of SHP1, and can be used as a normalization assay with our phospho-SHP1 Y564 kit. Because these phospho and total SHP1 assays are compatible, the two kits can be used in parallel on the same lysates. Many cancer cells overexpress checkpoint inhibitor ligands such as PD-L1. WebAkt1/2 kinase inhibitor indeed decreased pAkt in all four cell lines , while total Akt levels were not affected by the treatment. To determine whether induction of apoptosis caused … nuc matters handbook

AKT Blocks SIK1-Mediated Repression of STAT3 to Promote …

WebDec 16, 2009 · PIP3 recruits protein dependent kinase 1 (PDK1) and AKT, also known as protein kinase B (PKB), to the plasma membrane where AKT is phosphorylated at Thr308 by PDK1 and at Ser473 by mTOR complex 2 (TORC2) . AKT1 can also be phosphorylated at Ser473 by DNA-PK in the nucleus . AKT occupies a key regulatory node in the PI3K … WebThe FastScan™ Phospho-Akt (Ser473) ELISA Kit detects endogenous levels of Akt when phosphorylated at Ser473 as shown in Figure 1. This kit detects proteins from the … WebThe Alpha SureFire® Ultra™ HV Multiplex p-AKT 1/2/3 615 (Eu) assay kit is used to measure the phosphorylation of AKT1/2/3 (Ser473) plus another target of choice from the AlphaLISA SureFire ... Akt (1/2/3) at the phospho-Ser473 epitope, and one at a … nucly signature overlay bundle

AlphaLISA® SureFire Ultra™ HV Multiplex - PerkinElmer

Page 5 - Phospho-proteins - PerkinElmer

WebAKT, also known as protein kinase B, is an important regulator of numerous cellular processes including cell survival, glucose metabolism, transcription, and apoptosis. AKT … Web(Ser473) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Akt1 (Ser473) in human, rat and mouse cells. Please visit www.cellsignal.com for a complete listing of recommended companion products. #7134C PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit Species Cross-Reactivity: H, M, R … nuc med brain perfusion imagingWebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin … nine sovereigns at windsor

"WebApr 14, 2024 · Notably, when proline in the 14-3-3 binding motif was replaced with an alanine (P436A), the 14-3-3 recognition of SIK1 was profoundly attenuated (Supplementary Fig. S4G). Together, these data suggest that AKT-mediated SIK1 phosphorylation enhances SIK1 binding with 14-3-3, which in turn increases the rate of SIK1 transfer to the cytoplasm. " - Phospho-akt1/2/3 ser473 cellular kit

Phospho-akt1/2/3 ser473 cellular kit

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer

WebThe phospho-EGFR assay kit is designed for the cell-based quantitative detection of Tyr1068 phosphorylation on EGFR. Mutations that lead to an overexpression or hyperactivity of EGFR, also known as ErbB1 or HER1, are associated with a number of cancers, such as lung, colorectal, prostate, glioblastoma cancer, etc.

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WebThe phospho-AKT1 (Ser473) kit is designed for robust quantification of AKT1 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), Akt is an oncogene … LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

WebThe protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents Product specifications Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs. Introduction to product validation WebPhospho-AKT1 (Ser473) Antibody (700392) in ICC/IF Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature.

WebPhospho-Akt (Ser473) Rabbit mAb has been coated on the microwells. After incubation with cell lysates, phospho-Akt (Ser473) protein is captured by the coated antibody. Following extensive washing, Akt1 Mouse Antibody is added to detect the captured phospho-Akt1 (Ser473) protein. WebAlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer Supplier: Perkin Elmer Harmonized Code US-3822005090 The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Order Now …

WebMMP1 Antibody SKU: A...

WebPhospho-AKT1/2/3 (Ser473) cellular kit HTRF® Kit Diabetes and metabolic disorders A portfolio to tackle in-depth diabetes and metabolic disorders studies Explore Phospho-AKT1/2/3 (Ser473) control lysate HTRF® Control lysate Phospho-AKT1/2/3 (Thr308) cellular kit HTRF® Kit Phospho-AKT1/2/3 (Thr308) control lysate HTRF® Control lysate Guides nuc med/dx radiopharmaceuticalsWebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an oncogene playing a key role in controlling apoptosis, cell proliferation, transcription, cell migration, and glucose metabolism. nine space invadersWebThe Advanced Phospho-ERK assay kits are designed for the robust and highly sensitive quantification of ERK phosphorylation on Thr202/Tyr204 as an MAPK pathway readout. The simple mix-and-read protocol eliminates all wash steps for faster analysis and high quality output. The Total-ERK1/2 assay is compatible with the buffers from the Phospho or ... nuc med certificationWebphospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent Positive Control Cell Lysate (lyophilized) Detection Reagent (TMB Substrate) Stop Solution Plate seal Description … nucly sign inWebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an … nucmedicum bad schwartauWebSep 12, 2015 · Inhibition CellularAkt semisyntheticviridins epidermalgrowth factor–dependent activation HT-29colon cancer cells Westernhybrid- ization using anti-phospho-Ser 473 -Akt antibody (Fig. 2A). Quantitation phospho-Ser473 totalAkt (Fig. 2B) gave IC 50 values (FSE) 2.2nmol/L, PX-867 3.5nmol/L, nuc med examsWebPhospho-Akt (Ser473) Kit For use with human, mouse, rat, and non-human primate cell lysates and tissue lysates. This package insert must be read in its entirety before using … nuc med hemangioma protocol