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Peasy®-blunt cloning vector

WebpEASY® -Blunt Zero Cloning Kit 目录号： CB501-01 单 价： ￥780 规格： 20 rxns 60 rxns 数量： - + 说明书 载体信息 质检报告 立即下单 加入购物车 产品详情介绍 该载体通过自杀基 … WebPeasy Blunt Cloning Vector, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …

Genome editing in rice and wheat using the CRISPR/Cas system

WebSep 8, 2024 · The PCR product was inserted into the pEASY-Blunt Simple Cloning vector using ClonExpress ... Since the pMDC123 vector has a Bar resistance gene that makes it resistant to the herbicide glufosinate, 1.0 mg·L −1 of glufosinate-ammonium was used to pick out the transformants. The shoots grown back were planted in 1/2 Murashige and … WebFeb 15, 2024 · PCR产物进行胶回收后，连接至pEASY-Blunt Zero Cloning Kit克隆载体，转化大肠杆菌感受态DH5α中，挑取单克隆测序。 ... 表2 CRISPR/Cas9-PbGID1s 载体构建所需引物Tab.2 Primers for CRISPR/Cas9-PbGID1s vector construction. 表3 表达载体构建体系Tab.3 Expression vector construction system. hss bolt connection https://tfcconstruction.net

Gene Replacement by Intron Targeting with CRISPR-Cas9

WebThe “pEASY ” cloning vectors provide a highly efficient, 5 minutes, one-step cloning strategy for PCR product without the need of elimination of PCR buffers. Some of convenient … WebApr 13, 2024 · For probe labelling, specific 500 bp sequences of target genes were amplified from the complementary DNA (cDNA) of Wm82 with primers (Supplementary Data 9) and cloned to pEASY-Blunt cloning vector ... WebpEASY® - T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 minutes fast ligation of Taq-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. hobucken post office

拟南芥CPK6在钙离子信号转导过程中的生理功能研究_百度文库

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Peasy®-blunt cloning vector

pEASY Cloning Vectors - Cloning and Mutagenesis System

Web糙皮侧耳新831菌株为河南农业大学生命科学学院应用真菌研究室保藏；pEASY-blunt E1 expression kit、pEASY-blunt cloning kit、TransStart FastPfu DNA polymerase购自北京全式金生物技术有限公司；E.coliDH5α、E.coliBL21（DE3）购自北京博迈德基因技术有限公司；RNA trizol plus购自大连宝 ... Web1.2 细胞、菌体及载体 本试验使用Vero细胞由华南农业大学国家生猪种业工程技术研究中心猪病防控研究室保存；克隆宿主菌Trans5α化学感受态细胞和pEASY-Blunt Simple Cloning Kit®克隆载体，均购自北京全式金生物技术有限公司。

Peasy®-blunt cloning vector

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WebAug 9, 2024 · The pJET1.2/blunt cloning vector is a component of the CloneJET PCR Cloning Kit (Catalogue number: K1231), and it is not sold individually. It contains an eco47IR gene that encodes for a restriction enzyme Eco 47I that is harmful to the common laboratory E. coli strains used in molecular cloning. Web本发明属于植物基因工程领域，具体涉及一种控制水稻窄叶基因NAL13的分离克隆、功能验证，及其在水稻育种中的应用。本发明公开了水稻窄叶基因NAL13基因，其核苷酸序列如SEQ ID NO：1所示；其用途为改良禾本科植物株型；使植株的叶片变窄。

WebEasyTaq PCR Supermix DNA聚合酶、pEASY-Blunt Simple Cloning Vector购自全式金公司；其他化学试剂购自Sigma公司。 2 结果与分析 2.1 拟南芥cpk6突变体纯合鉴定 为了获得纯合突变体，用SDS方法提取Col-0和cpk6幼嫩叶子的总DNA，利用T-DNA特异引物LBb1.3和CPK6基因特异引物LP和RP进行PCR ... WebMar 27, 2024 · The pEASY-blunt simple cloning vector containing CRKII was digested with Hin dIII and Bam HI. The cleaved insert was ligated into a pcDNA3.1/V5-HisB vector (Shanghai CPG Biotech Co., Ltd., Shanghai, China) with T4 …

WebThe Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room … WebCloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but no multiple cloning site. Sequence …

WebMay 28, 2024 · A 2798 bp (− 2518 bp ~ + 280 bp; translation initiation site "ATG" was numbered as + 1) region of Ppd-D1b promoter was amplified from the wheat cultivar Chinese spring genomic DNA and cloned into pEASY-Blunt Cloning vectors, which was confirmed by sequencing.

WebpEASY®-Blunt Simple TOPO Cloning Kit. pEASY®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of the pEASY®-Blunt Cloning Vector.It is designed for cloning and sequencing blunt-end PCR products. Suggested DNA Polymerases include TransStart® FastPfu FLY and TransStart® KD Plus Ultra-HiFi DNA Polymerases. hss bohrer wikipediaWebelectrophoresis and ligated it into pEASY®-Blunt Zero Cloning Vector (TransGen Biotech Beijing Co., LTD). Then, the recombinant plasmids transformed into the resistant competent cell. The positive clones were screened and cultured in LB (10 mL) with ampicillin for 5 h. The recombinant plasmids were obtained by plasmid hobuck flats trailWebThe aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once … hobuck beach washington 98357WebSep 1, 2024 · Removal of the MCS fragment from the pJET1.2M plasmid produces the pJET1.2/blunt cloning vector ready for gene cloning. The self-made pJET1.2/blunt cloning vector exhibited a... hss bondWebpEASY Cloning Vectors pEASY® -Blunt Cloning Kit Catalog Number：CB101-01 Price：$89.02 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information … hss boltonWebligated to the pEasy-Blunt vector, and the resulting plasmids were then digested with restriction enzymes indicated in Table S2.The gel purified fragments were then ligated to the pETDuet-1 vector that had been digested with the same restriction enzymes to construct the plasmids for the expression of Aldo, DHAD, ALS and ALDC. hss bone age shorthandWebMay 7, 2024 · D型产气荚膜梭菌C60-2株、D型产气荚膜梭菌C60-2株天然毒素、D型产气荚膜梭菌抗毒素以及pET-30a(+)表达载体为本实验室保存；1.5～2.0 kg普通级健康日本大耳白兔和16～18 g ICR小鼠购自北京维通利华实验动物技术有限公司；pEASY-Blunt Cloning Vector、感受态细胞Top10和BL21(DE3 ... hss bone age