SpletThe TOPO XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb). The kit uses the linearized and topoisomerase 1-activated pCR-XL-2-TOPO vector, which is c Splet20. feb. 2024 · The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR …

pCR4-TOPO Sequence and Map - SnapGene

SpletThe TOPO XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb). The kit uses the linearized and … SpletDownload pCR-XL-2-TOPO.dna file Download Plasmid Open in SnapGene SnapGene SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures Fast accurate construct design for all major molecular cloning techniques Validate sequenced constructs using powerful alignment tools pintle style fuel injector

TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO ... - US

SpletpCR4-TOPO. Parental sequencing vector for TOPO® TA cloning of PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. Try SnapGene for Free. Download … SpletpCR2.1 TOPO Cloning PCR fragments, 3931 bp, f1 & pUC ori, lacZα, Amp r Kan r, screening by blue white colonies Invitrogen pCRXL TOPO Cloning large PCR fragments (3–10 kb), 3519 bp, pUC ori, lacZα, Zeocin r Kan r, cedB lethal gene, screening by blue white colonies Invitrogen pKHKS403 SpletThe TOPO™ XL-2 Complete PCR Cloning Kit provides a highly efficient one-step method ("TOPO™ Cloning") for the cloning of extra long PCR products generated by the … pintle swivel hitch