Ghd7-cfp
WebGhd7.1 explained 50.2%, 45.3%, and 76.9% of phenotypic variation in PH, SPP, and HD, respectively. Ghd7.1 was precisely mapped to a 357-kb region on the basis of analysis … WebOur analysis revealed a regulatory network that may provide an approximation to the genetic constitution of the optimal heterosis observed in this hybrid. In this network, Ghd7 works …
Ghd7-cfp
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WebMar 1, 2011 · To assay the sub-cellular localization of GHD8, the coding sequence of GHD8 was fused to yellow fluorescent protein (YFP); in contrast, GHD7, which is a nuclear protein, was fused to cyan fluorescent protein (CFP). Both fluorescent proteins were individually driven by the constitutive 35S cauliflower mosaic virus promoter. WebGrant Programs Directorate DHS/FEMA. 5071 D Techworld Building. 500 C Street SW. , Washington, District of Columbia 20472 Phone: (1-800) 368-6498. Legal Disclaimer. This …
WebJan 31, 2024 · GHD7 and GHD8 are identified with a pleiotropic effect on grain number, plant height and heading date in rice. Both genes delayed flowering through inhibiting the … WebFeb 24, 2024 · Scale bar, 2 mm. (I) Subcellular localization of SID1. Full-length SID1 fused with green fluorescent protein (GFP); the nuclear protein Ghd7 fused with cyan fluorescent protein (CFP) served as a nuclear marker. The two proteins were co-expressed in rice protoplasts. A bright-field image and the merged image are shown on the right. Scale …
WebJan 1, 2024 · The vector 35S:GHD7:CFP was used as reference as its located in the nuclear. These two vectors were co-transformed into Arabidopsis protoplast for NnTT8 subcellular localization analysis according to the method described by Xiong et al. (2024). WebSep 1, 2024 · To determine the subcellular localization of AtWRKY47, we co-expressed 35 S:WRKY47-GFP (green fluorescent protein) with the nuclear marker 35 S:Ghd7-CFP (cyan fluorescent protein) in Arabidopsis protoplasts. Nuclear localization of AtWRKY47 was observed since GFP fluorescence completely overlapped with CFP signal (Fig. 2 e).
WebOur analysis revealed a regulatory network that may provide an approximation to the genetic constitution of the optimal heterosis observed in this hybrid. In this network, Ghd7 works as the core element, and three other genes, Ghd7.1, Hd1, …
WebThe nuclei were identified by co-expressing the nuclear marker Ghd7-CFP with both 35S:SPL13-GFP and 35S:GFP. Fluorescence images were acquired using a confocal laser scanning microscope (Leica TCS SP2, MRC Centre for Regenerative Medicine, The University of Edinburgh, Edinburgh, UK) after incubating the protoplasts at 28 °C for … robert harris munich pdfWebOct 1, 2024 · During Agrobacterium (Agrobacterium tumefaciens) infection, the translocated virulence proteins (VirD2, VirE2, VirE3, VirF and VirD5) play crucial roles. It is thought that, through protein–protein... robert harris mys michiganWebMay 28, 2014 · Three transient expression vectors were constructed: 35S::VirD5-YFP, 35S::VirD5C-YFP and 35S::VirD5N-YFP. The empty yellow fluorescent protein (YFP, as control) and the construct YFP vector plasmid DNA were used to cotransform rice protoplasts with 35S::Ghd7-CFP. The 35S::Ghd7-CFP was used as a nuclear … robert harris obituary indianaWebMar 1, 2011 · Ghd7, which encodes a CCT-domain-containing transcription factor, was reported to regulate rice grain yield, plant height, and heading date. The natural variation … robert harris novelistWebJan 7, 2015 · Ghd7.1 encodes a PRR37-like protein that represses flowering by suppressing the expression of the downstream genes Ehd1 and Hd3a under LD, greatly enhancing grain productivity 23. All these... robert harris munich reviewsWebEnhanced expression of Ghd7 under long-day conditions delays heading and increases plant height and panicle size. Natural mutants with reduced function enable rice to be … robert harris munich filmWebNational Center for Biotechnology Information robert harris obituary 2021