WebApr 2, 2024 · The optimized multiplex PCR was performed in a 20 μl volume, in which the reaction mixture contained 2 μl 10 × PCR buffer with 2 mM MgCl 2, 0.8 mM of each dNTP, four sets of primers (0.2 μM of each primer) (Table 1), 1 μl of cDNA template and 1 U Taq DNA Polymerase (Invitrogen™). PCR was carried out in the thermal cycler. WebThe FastStart ™ High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below +75 °C and are activated by heating to +95 °C for two minutes.
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High-fidelity PCR utilizes DNA polymerases that couple low misincorporation rates with proofreading activity to give faithful replication of the target DNA of interest. When is the fidelity of a DNA polymerase important? Fidelity is important for applications in which the DNA sequence must be correct after amplification. WebHigh-Fidelity PCR, Multiplex PCR, Specialty PCR, Routine PCR, PCR Reagents Supplied Properties & Usage Advantages and Features Related Products References Protocols Usage Guidelines Selection Charts Web … ers overview of retirement
Phanta Super-Fidelity DNA Polymerase P501
WebOffice hours: Monday to Friday: 8:30 a.m. - 5:00 p.m. Contact. Not sure if you need an appointment? Call us and we’ll help! This Investor Center: 800-543-2158 (office hours … WebHigh Fidelity PCR dNTPs included: no hotstart: no packaging pkg of 100 U (04340868001) pkg of 500 U (04340850001 [2 x 250 U]) manufacturer/tradename Roche parameter 72 °C optimum reaction temp. technique (s) PCR: suitable input purified DNA storage temp. −20°C PCR Reagents & Kits Description General description ersoyakif1 twitter