Cycloheximide chx -chase-degradation assays
WebJan 20, 2010 · Cycloheximide ( CHX) tolerance of proteasome mutants. a The percentage of cotyledon emergence was calculated after 10 days of growth in continuous light at 22°C on MS/2 media supplemented with the denoted doses of CHX. Data are presented as mean ± SEM ( n ≥ 50). Significance was assessed by a two-way ANOVA with Tukey’s post test. WebApr 12, 2024 · Cycloheximide assay HLF cells were transfected for 24 h with siUSP1 or siControl. Then, 100 μmol/L cycloheximide (CHX) was added to the culture medium, and cell lysates were collected 0, 2, 4, 6 ...
Cycloheximide chx -chase-degradation assays
Did you know?
WebCycloheximide was used at 50µg per mL in the cell culture media. For the control protein, my blot was just fine. It is a quite stable protein so I observed no variations in protein quantity over ... WebApr 2, 2012 · Cycloheximide, Protein synthesis inhibitor Description Protein synthesis inhibitor Biological description Protein synthesis inhibitor. Antifungal antibiotic. CAS Number 66-81-9 Chemical structure Properties Applications Images Protocols Datasheets and documents References (22) Customer reviews and Q&As
WebJul 21, 2024 · The CHX chase assay was conducted for 12 hours with following ranges : 0 hour - 1 hour - 2 hours - 4 hours - 8 hours and 12 hours. I tested a control protein (wild type) and a mutant version of... WebBackground. Cycloheximide (CHX; mol. wt. 281.35) is a beige colored, crystal powdered, water-soluble, semisynthetic compound, isolated as a by-product from beers of streptomycin-producing strain of a Streptomyces species. CHX, a popular inhibitor of protein synthesis, also stimulates glycogenolysis, gluconeogenesis, and ureogenesis; therefore ...
WebJul 14, 2024 · PurposeUbiquitin D (UBD) is a member of the ubiquitin-like modifier (UBL) family and is highly expressed in a variety of cancers including colorectal cancer (CRC). However, the mechanisms of its regulatory roles in CRC are largely elusive. In this study, we revealed the effect of UBD on the proliferation of CRC.MethodsThe expression of UBD in … WebMay 25, 2024 · On the basis of CFTS assays, we attempted to bind CHX at high excess in nonphysiological conditions (≥1 mM) to understand possible rearrangements in the E-site upon the inhibitor binding. Only at 1 mM was density for …
WebAfter 24 h of cycloheximide chase, only the mature glycosylated form was visible in the immunoblots of wild type (Fig. 1a) and the total VLDLR levels were reduced to ~50%. On the other hand, the...
WebJul 14, 2024 · Figure 4. CMP induces loss of viability in MDA-MB-231 in 3D spheroid assay, as reported by Incucyte® Cytotox Green reagent. MDA-MB-231 cells were seeded in the presence of Incucyte® Cytotox ... importance of government spendingWebNov 14, 2024 · The CHX chase assay was conducted for 12 hours with following ranges : 0 hour - 1 hour - 2 hours - 4 hours - 8 hours and 12 hours. I tested a control protein (wild type) and a mutant version of... literally guy tik tokWebCycloheximide chase assays are an experimental technique used in molecular and cellular biology to measure steady state protein stability. Cycloheximide is a drug that inhibits the elongation step in eukaryotic protein … importance of gps in logisticsWebNov 14, 2014 · Lipid Efflux Assays—HDL subfractions, LDL, and apoA-I were obtained from human serum by ultracentrifugation, ... To assess the trafficking of ABCG1 from the ER to the cell surface and late endosomes, cells were treated with cycloheximide (CHX) to block protein synthesis. As anticipated, CHX reduced cellular levels of ABCG1-GFP (Figure 6A ... importance of grading and reporting systemWebGeneral Description. • Cycloheximide is a semisynthetic compound showing an antifungal antibiotic activity. • Cycloheximide can inhibit protein synthesis in eukaryotic cells but not in prokaryotes by interacting directly with the translocase enzyme and interfering with the translocation step. importance of gps in surveyingWebBackground. Cycloheximide (CHX; mol. wt. 281.35) is a beige colored, crystal powdered, water-soluble, semisynthetic compound, isolated as a by-product from beers of streptomycin-producing strain of a Streptomyces species. CHX, a popular inhibitor of protein synthesis, also stimulates glycogenolysis, gluconeogenesis, and ureogenesis; therefore ... importance of gps trackerWebAug 9, 2024 · We then investigated the effects of TRIM28 on NLRP3 protein degradation by cycloheximide (CHX) chase assay. Trim28 deficiency markedly promoted NLRP3 protein degradation (Fig. 4d, e ).... importance of gps