WebNov 9, 2024 · Immunohistochemistry on brain sections. Immunohistochemistry for cFos, Ki67, BrdU, Dcx, NeuN and S100ß in brain sections was performed following published procedures from our lab 25,26,27. For ... WebJul 20, 2024 · c-Fos Fluorescent Immunohistochemistry Protocol. Day 1: 1) 3 x 5 min wash in PBS. 2) 30 min wash 10% PBS-Triton X (TX) with 3% horse serum. 3) Add primary antibody for 3-day incubation period …
Anti-c-Fos antibody [2H2] (ab208942) Abcam
WebMar 1, 2014 · For visualization of cfos in NPY GFP neurons or POMC and NPY GFP neurons in the ARC (n = 6 per group), we used a single-label immunohistochemistry approach in which cfos or POMC was stained, as indicated above except that fluorescent secondary antibodies were used (Alexa Fluor goat anti-rabbit 594 antibody, A-11012; … WebMar 27, 2024 · For cell lines, you may use immunocytochemistry (ICC) which is performed on sample of intact cells. ICC is a common laboratory assay that can confirm the expression and location of target peptides... bubble shooter family pack
c-fos Immunohistochemistry Protocol - figshare
WebThe immediate-early-gene product c-Fos is a well known marker of neuronal … WebJan 28, 2014 · A dramatic bradycardia is induced by underwater submersion in vertebrates. The location of parasympathetic preganglionic cardiac motor neurons driving this aspect of the diving response was investigated using cFos immunohistochemistry combined with retrograde transport of cholera toxin subunit B (CTB) to double-label neurons. After … WebWe isolated the brains, which were immunohistochemically stained for c-Fos as a biomarker for neuronal activation and assessed the expression profile of c-Fos in various sleep-arousal brain areas. Results: Unlike saline, spinosin markedly decreased c-Fos expression in the lateral hypothalamic area (LHA) as well as the locus coeruleus (LC). bubble shooter extreme gratuit