WebMar 1, 1999 · A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. ... Gene-specific primers were from commercially available amplimer sets (Clontech). WebThe miRCURY LNA miRNA Probe PCR System uses a single cDNA synthesis reaction for all amplifications, reducing pipetting and saving time and sample. A forward PCR primer and hydrolysis probe that are LNA-enhanced and miRNA-specific deliver highly sensitive miRNA quantification and specificity down to a single-nucleotide sequence difference.
Technique for strand-specific gene-expression analysis …
WebDownload scientific diagram Schematic view of TOGA. Poly(A)-selected RNA serves as template for double-strand cDNA synthesis by using a pool of Not I-containing biotinylated (B) primers ... WebReactions using random hexamers and/or oligo-dT primers produce cDNA that is a mixture of various RNA species in the sample. The cDNA synthesis reaction can be scaled up to … carburetors and caffeine
miRNA Quantification - Qiagen
WebThe SuperScript™ First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly (A)+ or total RNA. The system can be used with as little as 1 ng or as much as 5 µg … WebThe mix is compatible with random primers, oligo dT primers, and gene-specific primers, enabling maximum cDNA synthesis flexibility. Learn more here. In comparison to the LunaScript kit version (NEB #E3010), this LunaScript product does not contain a No-RT Control Mix or nuclease-free water. More details can be found below: WebFirst-strand synthesis of cDNA utilizes either oligo(dT), random primers, or a combination of these strategy to prime and reversed transcription reaction. Priming a reaction with oligo(dT) initiations the synthesis preferred at and 3’ end of aforementioned RNA fragment. carburetor scoops for boats